Publications

Canadian Spine Society
Adeesya Gausper
Lindsay M Andras
Ken D. Illingworth
David L. Skaggs
Rachelle Imbeault
Justin Dufresne
Sylvain Deschênes
Marjolaine Roy-Beaudry
Jack Legler
L. Benaroch
Olivia Serhan
Draydon Cheng
Debra Bartley
Patrick Thornley
Khaled Skaik
Genevieve Belanger
Alexandra Stratton
Coyle Matthew
Stephen P. Kingwell
Eve C. Tsai … (see 355 more)
Eugene K. Wai
Hannah Fonteyne
S. Hryniuk
Eric Parent
Eric Huang
K. Stampe
Marie J Beaulieu
Monica Chan
Gloria Thevasagayam
Gabriela Marino-Merlo
Zaid Salaheen
A. Malvea
Leeor Yefet
Ali Moghaddamjou
Sam Molot-Toker
Eisha Christian
Jennifer L Quon
P. B. Dirks
James M. Drake
James T. Rutka
Abhaya V. Kulkarni
Reinhard Zeller
George M. Ibrahim
Julie Joncas
Soraya Barchi
Stefan Parent
Karim Aboelmagd
Archana Sivakuganandan
Amna Zulfiqar
Anne Murphy
Stanley Moll
Julia Sorbara
Brett Rocos
Mark Camp
Geoffrey K. Shumilak
Jalen Dansby
Andrew Chan-Tai-Kong
David E. Lebel
Daisy A Lu
Monique Clar
M. F. Al-Shakfa
Parham Rasoulinejad
Firoz Miyanji
Karim Kantar
Timothy P. Carey
Ravi Ghag
Brent Lanting
Zeeshan Sardar
Saumayajit Basu
Manish Gupta
Abdullah T. Eissa
So Kato
Lawrence G. Lenke
Kristen Jones
Saumyajit Basu
Michael P Kelly
Justin Smith
S. Strantzas
Yong Qiu
Ferran Pellise
A. Alanay
Nasir A. Quraishi
R. Gray
G. Yoshida
Amer Aziz
Jennyfer Paulla Galdino Chaves
Brian Hsu
Stone Sima
Bhisham Singh
Vinay Kulkarni
Ashish D. Diwan
Taryn Ludwig
Farbod Moghaddam
Mina Aminghafari
May Choi
Eliana Seider
Lauren Daunt
Vanessa Vashishth
Ali Ahmadi
P. Brzozowski
Asra Toobaie
Renan R. Fernandes
Anthony V. Perruccio
Amir Mishreky
Mark Alexander MacLean
Lisa Julien
G. Patriquin
Jason Leblanc
Ryan Greene
J. Alant
Sean Barry
R. Glennie
Sean D. Christie
Greg MacIntosh
Daniel P Smith
Erin Bigney
Jeffrey Hebert
Eden Richardson
Neil Manson
Edward Abraham
Abdullah Zein
Kyra Holt
Hannah Isaac
Jillian Kearney
Chris Small
Abdullah A.S.M. AlDuwaisan
V. Smith
Tara Whittaker
Denise Eckenswiller
Elias Soumbasis
Robert Tanguay
Celina Nahanni
Tiffany Lung
James J Young
Chloe N Cadieux
Jin Tong Du
Raja Rampersaud
Andrew Glennie
Cynthia Dunning
E. Jones
William Oxner
Kaike Lobo
P. Łajczak
Cláudia Santos
Numa Rajab
Rafael Oliveira
Y. Silva
R. Barbosa
Aazad Abbas
Gurjovan Sahi
Michael B Johnson
Edward Buchel
Jay Toor
Ronit Kulkarni
Melanie Bertolino
Chase Walton
Gabriella Rivas
John Glaser
Charles Reitman
James Lawrence
Robert A. Ravinsky
Mohammed Ali Alvi
Avery B. Nathens
Eva Yuan
Yingshi He
Francois Mathieu
Michael C. Sklar
Samuel Yoon
Luke Reda
Hussain Shakil
S. Sadiqi
S. Muijs
Charlotte Dandurand
Marcel Dvorak
F. C. Oner
Vivian Huong Hoang Thien Le
Pascal Mputu Mputu
Francis Bernard
Yiorgos Alexandros Cavayas
H. Hong
D. Kurban
Tianyu Yang
Nader Fallah
Christiana L Cheng
Suzanne Humphreys
Vanessa K. Noonan
Dana El-mughayyar
Colleen O’Connell
Husain Shakil
Zixuan Hu
Christopher W. Smith
Hui M Ling
Zakariya M Khan
Ervin Sejdic
Errol Colak
Christopher Witiw
Maude Duguay
Juan David Cifuentes Hernandez
Jean-Marc Mac-Thiong
Antoine Dionne
Natan Bensoussan
Andréane Richard-Denis
Louis Carrier
Jocelyn Blanchard
Bernard LaRue
Ariane Paquette
Yan Gabriel Morais David Silva
Christopher Nielsen
Vaidehi Bhatt
Stephen J. Lewis
Y. Raja Rampersaud
Brent Rosenstein
Chanelle Montpetit
Nicolas Vaillancourt
Geoffrey Dover
Christina Weiss
Lee Ann Papula
Antonys Melek
Maryse Fortin
A. Fazlollahi
Uri Barak
Samira Kalhor
Joshua Nguyen
Carlo Santaguida
Ruheksh Raj
Kyle Rogan
Allison Marchuk
Erin Barrett
Anand Masson
Brandy Pratt
Danielle Michaud
Kateryna Skyrda
Sierra Simms
Brandon Herrington
Fawaz Siddiqi
Kevin Gurr
Mathieu Chayer
P-J Arnoux
Jeremy Rawlinson
Olumide Aruwajoye
Carl-Eric Aubin
Hussein Samhat
K. Pedro
A. A. Pirshahid
Genevieve Gore
K. Filion
Oliver Lasry
Jordan J. Levett
Nathan Evanview
G. Mcintosh
Nadav Rinott
Mathieu Laflamme
Andy Liu
Alexander Tuchman
Christopher Mikhail
Vivien K. Chan
James McDonald
Julien Zaldivar
G. Lonjon
Matthieu Vassal
Alexandre Dhenin
Alexis Perez
Martin Dupuy
V. Challier
J. Castelain
S. Ghailane
Matthieu Campana
Jonathan Lebhar
Gilles Guerin
Nicolas Pellet
Yann Sabah
T. Chevillotte
A. Darnis
Joseph Cristini
F.X. Ferracci
J. Delambre
Steffen Queinnec
Alexandre Delmotte
Paulo Marinho
R. Gauthé
P. Hannequin
Vianney Gilard
Jean Meyblum
Alexis Perrin
Raphaël Pietton
Nicolas Lonjon
Antoine Gennari
Ahmed Essa
Michael Craig
Kalum Ost
W. Bradley Jacobs
Peter Lewkonia
Fred Nicholls
Michael Yang
Julien-Cohen Adad
Isaac Wangai
Andrew Nataraj
Osman Hojanepesov
Matthew Skarsgard
Nathan Evaniew
Jérôme Paquet
Perry Dhaliwal
Najmedden Attabib
Chris Bailey
Jefferson R. Wilson
Daniel Kurtz
P. Phan
Christopher Sun
Newton Cho
Abdul Al-Shawwa
Bradley W. Jacobs
Jacques Bouchard
Steven Casha
Stephan duPlessis
Alex Soroceanu
Ganesh Swamy
Kenneth C. Thomas
Julien-Cohen Adad
David W. Cadotte
Landon J Hansen
Stephen Yip
Nicolas Dea
Chetan Bettegowda
Laurence D. Rhines
Daniel M. Sciubba
James M. Schuster
Stefano Boriani
M. Clarke
Paul M. Arnold
Anne Versteeg
Michael H. Weber
R. de la Garza Ramos
John Shin
Markian Pahuta
A. Luzatti
A. Disch
A. Gasbarrini
Jorrit‐jan Verlaan
William G J Teixeira
Ilya Laufer
Á. Lazáry
Dean Chou
Z. Gokaslan
Addisu Mesfin
Tony Goldschlager
C. Netzer
J. O'Toole
Ori Barzilai
Norio Kawahara
Naresh Kumar
Jeremy J. Reynolds
Rory Goodwin
Jetan Badhiwala
A. Sahgal
Michael G. Fehlings
Alex Kiss
Donald A. Redelmeier
William Chu Kwan
Nikolaus Koegl
Charles G. Fisher
Raphaële Charest-Morin
Alexandra Rocha
Matthew Renaud
Jennifer C. Urquhart
Supriya Singh
Marco Pérez Caceres
Omer Ahmed
Véronique Freire
Jesse Shen
Fidaa Al-shakfa
Danielle Boule
Z. Wang
Christopher S. Lozano
Armaan Malhotra
Vishwathsen Karthikeyan
2025-12-11
Canadian journal of surgery. Journal canadien de chirurgie (published)
doi.org
Canadian Spine Society
Adeesya Gausper
Lindsay M Andras
Ken D. Illingworth
David L. Skaggs
Rachelle Imbeault
Justin Dufresne
Sylvain Deschênes
Marjolaine Roy-Beaudry
Jack Legler
Lee Benaroch
Olivia Serhan
Draydon Cheng
Debra Bartley
Patrick Thornley
Khaled Skaik
Genevieve Belanger
Alexandra Stratton
Coyle Matthew
Stephen P. Kingwell
Eve C. Tsai … (see 355 more)
Eugene K. Wai
Hannah Fonteyne
S. Hryniuk
Eric Parent
Eric Huang
K. Stampe
Marie J Beaulieu
Monica Chan
Gloria Thevasagayam
Gabriela Marino-Merlo
Zaid Salaheen
A. Malvea
Leeor Yefet
Ali Moghaddamjou
Sam Molot-Toker
Eisha Christian
Jennifer L Quon
P. B. Dirks
James M. Drake
James T. Rutka
Abhaya V. Kulkarni
Reinhard Zeller
George M. Ibrahim
Julie Joncas
Soraya Barchi
Stefan Parent
Karim Aboelmagd
Archana Sivakuganandan
Amna Zulfiqar
Anne Murphy
Stanley Moll
Julia Sorbara
Brett Rocos
Mark Camp
Geoffrey K. Shumilak
Jalen Dansby
Andrew Chan-Tai-Kong
David E. Lebel
Daisy A Lu
Monique Clar
M. F. Al-Shakfa
Parham Rasoulinejad
Firoz Miyanji
Karim Kantar
Timothy P. Carey
Ravi Ghag
Brent Lanting
Zeeshan Sardar
Saumayajit Basu
Manish Gupta
Abdullah T. Eissa
So Kato
Lawrence G. Lenke
Kristen Jones
Saumyajit Basu
Michael P Kelly
Justin Smith
S. Strantzas
Yong Qiu
Ferran Pellise
A. Alanay
Nasir A. Quraishi
R. Gray
G. Yoshida
Amer Aziz
Jennyfer Paulla Galdino Chaves
Brian Hsu
Stone Sima
Bhisham Singh
Vinay Kulkarni
Ashish D. Diwan
Taryn Ludwig
Farbod Moghaddam
Mina Aminghafari
May Choi
Eliana Seider
Lauren Daunt
Vanessa Vashishth
Ali Ahmadi
P. Brzozowski
Asra Toobaie
Renan Rodrigues Fernandes
Anthony V. Perruccio
Amir Mishreky
Mark Alexander MacLean
Lisa Julien
Glenn Patriquin
Jason Leblanc
Ryan Greene
J. Alant
Sean Barry
R. Glennie
Sean D. Christie
Greg MacIntosh
Daniel P Smith
Erin Bigney
Jeffrey Hebert
Eden Richardson
Neil Manson
Edward Abraham
Abdullah Zein
Kyra Holt
Hannah Isaac
Jillian Kearney
Chris Small
Abdullah A.S.M. AlDuwaisan
V. Smith
Tara Whittaker
Denise Eckenswiller
Elias Soumbasis
Robert Tanguay
Celina Nahanni
Tiffany Lung
James J Young
Chloe N Cadieux
Jin Tong Du
Raja Rampersaud
Andrew Glennie
Cynthia Dunning
Emma Jones
William Oxner
Kaike Lobo
P. Łajczak
Cláudia Santos
Numa Rajab
Rafael Oliveira
Y. Silva
R. Barbosa
Aazad Abbas
Gurjovan Sahi
Michael B Johnson
Edward Buchel
Jay Toor
Ronit Kulkarni
Melanie Bertolino
Chase Walton
Gabriella Rivas
John Glaser
Charles Reitman
James Lawrence
Robert Ravinsky
Mohammed Ali Alvi
Avery B. Nathens
Eva Yuan
Yingshi He
Francois Mathieu
Michael C. Sklar
Samuel Yoon
Luke Reda
Hussain Shakil
S. Sadiqi
S. Muijs
Charlotte Dandurand
Marcel Dvorak
F. C. Oner
Vivian Huong Hoang Thien Le
Pascal Mputu Mputu
Francis Bernard
Yiorgos Alexandros Cavayas
H. Hong
D. Kurban
Tianyu Yang
Nader Fallah
Christiana L Cheng
Suzanne Humphreys
Vanessa K. Noonan
Dana El-mughayyar
Colleen O’Connell
Husain Shakil
Zixuan Hu
Christopher W. Smith
Hui M Ling
Zakariya M Khan
Ervin Sejdic
Errol Colak
Christopher Witiw
Maude Duguay
Juan David Cifuentes Hernandez
Jean-Marc Mac-Thiong
Antoine Dionne
Natan Bensoussan
Andréane Richard-Denis
Louis Carrier
Jocelyn Blanchard
Bernard LaRue
Ariane Paquette
Yan Gabriel Morais David Silva
Christopher Nielsen
Vaidehi Bhatt
Stephen J. Lewis
Y. Raja Rampersaud
Brent Rosenstein
Chanelle Montpetit
Nicolas Vaillancourt
Geoffrey Dover
Christina Weiss
Lee Ann Papula
Antonys Melek
Maryse Fortin
A. Fazlollahi
Uri Barak
Samira Kalhor
Joshua Hien Nguyen
Carlo Santaguida
Ruheksh Raj
Kyle Rogan
Allison Marchuk
Erin Barrett
Anand Masson
Brandy Pratt
Danielle Michaud
Kateryna Skyrda
Sierra Simms
Brandon Herrington
Fawaz Siddiqi
Kevin Gurr
Mathieu Chayer
P-J Arnoux
Jeremy Rawlinson
Olumide Aruwajoye
Carl-Eric Aubin
Hussein Samhat
K. Pedro
A. A. Pirshahid
Genevieve Gore
K. Filion
Oliver Lasry
Jordan J. Levett
Nathan Evanview
G. Mcintosh
Nadav Rinott
Mathieu Laflamme
Andy Liu
Alexander Tuchman
Christopher Mikhail
Vivien K. Chan
James McDonald
Julien Zaldivar
G. Lonjon
Matthieu Vassal
Alexandre Dhenin
Alexis Perez
Martin Dupuy
V. Challier
J. Castelain
S. Ghailane
Matthieu Campana
Jonathan Lebhar
Gilles Guerin
Nicolas Pellet
Yann Sabah
T. Chevillotte
A. Darnis
Joseph Cristini
F.X. Ferracci
J. Delambre
Steffen Queinnec
Alexandre Delmotte
Paulo Marinho
R. Gauthé
P. Hannequin
Vianney Gilard
Jean Meyblum
Alexis Perrin
Raphaël Pietton
Nicolas Lonjon
Antoine Gennari
Ahmed Essa
Michael Craig
Kalum Ost
W. Bradley Jacobs
Peter Lewkonia
Fred Nicholls
Michael Yang
Julien-Cohen Adad
Isaac Wangai
Andrew Nataraj
Osman Hojanepesov
Matthew Skarsgard
Nathan Evaniew
Jérôme Paquet
Perry Dhaliwal
Najmedden Attabib
Chris Bailey
Jefferson R. Wilson
Daniel Kurtz
P. Phan
Christopher Sun
Newton Cho
Abdul Al-Shawwa
Bradley W. Jacobs
Jacques Bouchard
Steven Casha
Stephan duPlessis
Alex Soroceanu
Ganesh Swamy
Kenneth C. Thomas
Julien-Cohen Adad
David W. Cadotte
Landon J Hansen
Stephen Yip
Nicolas Dea
Chetan Bettegowda
Laurence D. Rhines
Daniel M. Sciubba
James M. Schuster
Stefano Boriani
M. Clarke
Paul Arnold
Anne Versteeg
Michael H. Weber
R. de la Garza Ramos
John Shin
Markian Pahuta
A. Luzatti
A. Disch
A. Gasbarrini
Jorrit‐jan Verlaan
William G J Teixeira
Ilya Laufer
Á. Lazáry
Dean Chou
Z. Gokaslan
Addisu Mesfin
Tony Goldschlager
C. Netzer
J. O'Toole
Ori Barzilai
Norio Kawahara
Naresh Kumar
Jeremy J. Reynolds
Rory Goodwin
Jetan Badhiwala
A. Sahgal
Michael G. Fehlings
Alex Kiss
Donald A. Redelmeier
William Chu Kwan
Nikolaus Koegl
Charles G. Fisher
Raphaële Charest-Morin
Alexandra Rocha
Matthew Renaud
Jennifer C. Urquhart
Supriya Singh
Marco Pérez Caceres
Omer Ahmed
Véronique Freire
Jesse Shen
Fidaa Al-shakfa
Danielle Boule
Z. Wang
Christopher S. Lozano
Armaan Malhotra
Vishwathsen Karthikeyan
2025-12-11
Canadian journal of surgery. Journal canadien de chirurgie (published)
doi.org
Learning to Extract Context for Context-Aware LLM Inference
Minseon Kim
Lucas Caccia
Zhengyan Shi
Matheus Pereira
Marc-Alexandre Côté
Xingdi Yuan
Alessandro Sordoni
2025-12-11
ArXiv (preprint)
doi.org
arxiv.org
AI Models of Human Brain and Behaviour During Naturalistic Videogame Play
Lune Bellec
Yann Harel
François Paugam
André Cyr
Basile Pinsard
Julie Boyle
Slides of the presentation given at the Montreal Artificial Intelligence and Neuroscience (MAIN) conference, December 2025, by Lune Bellec. … (see more)A video recording of the presentation is available on youtube.
2025-12-11
Zenodo (unknown)
doi.org
Transformer Embeddings for Fast Microlensing Inference
Nolan Smyth
Laurence Perreault-Levasseur
Yashar Hezaveh
2025-12-11
ArXiv (preprint)
arxiv.org
arxiv.org
Critical Role of EEG Signals in Assessment of Sex-Specific Insights in Neurological Diagnostics via Machine Learning Approach
Mohammad-Javad Darvishi-Bayazi
Mohammad Sajjad Ghaemi
Irina Rish
Jocelyn Faubert
Abstract

Early detection and diagnosis of pathology are essential for efficient treatment and therapeutic … (see more)interventions. The emergence of Artificial Intelligence (AI) and deep machine learning techniques have demonstrated the promising capability of brain imaging data to predict various pathological diseases. However, plenty of diseases have imbalanced distribution across different sexes. Furthermore, the impact of sex-specific patterns and biomarkers in predicting diseases has remained unexplored as a fundamental subject matter to inform the treatment paradigms. This paper underscored the generalization and transferability of sex-related patterns in functional data, specifically Electroencephalogram (EEG) signals through Artificial Deep Neural Networks. We conducted training on a broad spectrum of EEG recordings involving participants ranging from 221 to 12,000, including healthy and pathological subjects. Our evaluation leveraged datasets from various sources and participant groups, featuring distribution shifts. While the artificial models demonstrated accurate sex detection on datasets without fine-tuning, their performance declined with significant distribution shifts. Furthermore, we explored the relationship between sex and pathology by visualizing salient features for target detection in distinct subgroups. Our findings revealed unprecedented insights into the negligible role of sex-specific patterns in pathology detection despite the presence of prominent and consistent patterns within sex groups. These results are essential for developing more robust and unbiased AI models for disease prediction and informing the treatment paradigms.

2025-12-09
Scientific Reports (published)
doi.org
SIDISH integrates single-cell and bulk transcriptomics to identify high-risk cells and guide precision therapeutics through in silico perturbation
Yasmin Jolasun
Kailu Song
Yumin Zheng
Jingtao Wang
Gregory Fonseca
David H. Eidelman
Jun Ding
Single-cell RNA sequencing (scRNA-seq) provides high-resolution insights into cellular heterogeneity but remains costly, restricting its use… (see more) to small cohorts that often lack comprehensive clinical data, reducing translational relevance. In contrast, bulk RNA sequencing is scalable and cost-effective but obscures critical single-cell insights. We introduce SIDISH, a neural network framework that integrates the granularity of scRNA-seq with the scalability of bulk RNA-seq. Using a variational autoencoder, deep Cox regression, and transfer learning, SIDISH identifies high-risk cell populations while enabling robust clinical predictions from large-cohort data. Its in silico perturbation module identifies therapeutic targets by simulating interventions that reduce high-risk cells associated with adverse outcomes. SIDISH also generalizes to spatial transcriptomics, identifying high-risk cells and mapping them within their native tissue microenvironment. Applied across diverse diseases, SIDISH establishes the link between cellular dynamics and clinical phenotypes, facilitating biomarker discovery and precision medicine. By unifying single-cell insights with large-scale clinical data, SIDISH advances computational tools for disease risk assessment and therapeutic prioritization, offering an integrative and scalable approach to precision medicine. SIDISH integrates single-cell and bulk RNA sequencing data using deep learning to identify high-risk cell populations and prognostic biomarkers, enabling in silico perturbations that could guide precision therapeutics and advance personalized medicine.
2025-12-09
Nature Communications (published)
doi.org
Conformal Selection for Efficient and Accurate Compound Screening in Drug Discovery
Tian Bai
Peng Tang
Yuting Xu
Vladimir Svetnik
Bingjia Yang
Abbas Khalili
Xiang Yu
Archer Y. Yang
In drug discovery, the reliability of compound screening based on manual assessments is compromised by potential bias, while existing method… (see more)s lack robust risk control measures. To address these challenges, we introduced conformal selection as an enhanced approach to optimize the compound screening process with balanced risks and benefits. Leveraging conformal inference, our approach constructs p-values for each candidate molecule to quantify statistical evidence for selection. The final selection of molecules is determined by comparing these p-values against thresholds derived from multiple testing principles. Our approach offers rigorous control over the false discovery rate, ensuring validity independent of dataset size and requiring minimal assumptions. By avoiding the estimation of prediction errors required in previous approaches, our method achieves higher accuracy (power), thereby improving the ability to identify promising candidates. Furthermore, our method demonstrates superior computational efficiency. We validate these advantages through numerical simulations on real-world datasets.
2025-12-08
Journal of Chemical Information and Modeling (unknown)
doi.org
Distributed Combined Space Partitioning and Network Flow Optimization: an Optimal Transport Approach
Théo Laurentin
Patrick Coirault
Emmanuel Moulay
Antoine Lesage-Landry
Jerome Le Ny
2025-12-08
2025 IEEE 64th Conference on Decision and Control (CDC) (published)
doi.org
arxiv.org
Generalized certainty equivalence based policies in partially observable systems
Berk Bozkurt
Aditya Mahajan
Ashutosh Nayyar
Yi Ouyang
In this paper, we present a generalization of the certainty equivalence principle of stochastic control. One interpretation of the classical… (see more) certainty equivalence principle for linear systems with output feedback and quadratic costs is as follows: the optimal action at each time is obtained by evaluating the optimal state-feedback policy of the stochastic linear system at the minimum mean square error (MMSE) estimate of the state. Motivated by this interpretation, we consider certainty equivalent policies for general (non-linear) partially observed stochastic systems and allow for any state estimate rather than restricting to MMSE estimates. In such settings, the certainty equivalent policy is not optimal. For models with Lipschitz cost and dynamics, we derive upper bounds on the sub-optimality of certainty equivalent policies in terms of expected error of the proposed estimator. We present several examples to illustrate the results.
2025-12-08
IEEE Conference on Decision and Control (published)
doi.org
Reference radiation selection is confirmed as a significant source of relative biological effectiveness variation for neutrons.
Laura C Paterson
Stephen Pecoskie
Farrah Norton
Norma Ybarra
J. Kildea
Richard B Richardson
2025-12-08
International Journal of Radiation Biology (published)
doi.org
Cell-type specific transcriptional modulation by psilocybin induces sustained plasticity in mouse medial prefrontal cortex
Delong Zhou
Heike Schuler
Vedrana Cvetkovska
Juliet Meccia
Ashot S. Harutyunyan
Jiannis Ragoussis
Rosemary C. Bagot
Ashot S. Harutyunyan
Jiannis Ragoussis
Danilo Bzdok
Rosemary C. Bagot
Despite enormous interest in psychedelics for psychiatric interventions, potential underlying biological mechanisms remain unclear. Here, we… (see more) confirm that a single dose of psilocybin increases synaptic transmission in mouse medial prefrontal cortex. Using scRNA-sequencing, we identify cell-type specific mechanisms of sustained neuroplastic effects. We show that, 24h post-psilocybin, expression of plasticity-related genes is increased in excitatory neurons and that transcription in a type of deep layer near projecting neuron, L5/6 NP, is robustly altered. Analyzing receptor expression patterns reveals that this cell-type specificity does not align with 5-HT 2A expression but aligns with 5-HT 2C expression patterns. Further, multivariate analyses identify psilocybin-induced gene expression patterns in L5/6 NP neurons predict 5-HT 2C , but not 5-HT 2A , transcript levels. Pharmacologic manipulation with a 5-HT 2C antagonist attenuates the post-acute sustained effect of psilocybin on synaptic transmission, highlighting 5-HT 2C signaling and L5/6 NP neurons as key mediators of psychedelic drug action’s sustained neuroplastic effects in mPFC.
2025-12-07
bioRxiv (preprint)
doi.org