Antoine Soulé

Unbiased characterization of COVID-19 endotypes leads to prognostication of high-risk individuals using routine blood tests

William Ma

Antoine Soulé

Catherine Allard

Madeleine Durand

Karine Tremblay

Simon Rousseau

Amin Emad

Unsupervised proteomic analysis identified biologically coherent endotypes that advance understanding of acute lung injury in COVID‑19 and… (voir plus) support improved diagnostic and prognostic strategies.

2026-03-13

Communications Medicine (publié)

doi.org

Divergent responses to SARS-CoV-2 infection in bronchial epithelium with pre-existing respiratory diseases

Justine Oliva

Manon Ruffin

Claire Calmel

Aurélien Gibeaud

Andrés Pizzorno

Clémence Gaudin

Solenne Chardonnet

Viviane de Almeida Bastos

Manuel Rosa-Calatrava

Antoine Soulé

Amin Emad

Simon Rousseau

Harriet Corvol

Olivier Terrier

Loïc Guillot

2025-02-28

iScience (publié)

doi.org

Circulating IL-17F, but not IL-17A, is elevated in severe COVID-19 and leads to an ERK1/2 and p38 MAPK-dependent increase in ICAM-1 cell surface expression and neutrophil adhesion on endothelial cells

Jérôme Bédard-Matteau

Antoine Soulé

Katelyn Yixiu Liu

Lyvia Fourcade

Douglas D. Fraser

Amin Emad

Simon Rousseau

Severe COVID-19 is associated with neutrophilic inflammation and immunothrombosis. Several members of the IL-17 cytokine family have been as… (voir plus)sociated with neutrophilic inflammation and activation of the endothelium. Therefore, we investigated whether these cytokines were associated with COVID-19. We investigated the association between COVID-19 and circulating plasma levels of IL-17 cytokine family members in participants to the Biobanque québécoise de la COVID-19 (BQC19), a prospective observational cohort and an independent cohort from Western University (London, Ontario). We measured the in vitro impact of IL-17F on intercellular adhesion molecule 1 (ICAM-1) cell surface expression and neutrophil adhesion on endothelial cells in culture. The contribution of two Mitogen Activated Protein Kinase (MAPK) pathways was determined using small molecule inhibitors PD184352 (a MKK1/MKK2 inhibitor) and BIRB0796 (a p38 MAPK inhibitor). We found increased IL-17D and IL-17F plasma levels when comparing SARS-CoV-2-positive vs negative hospitalized participants. Moreover, increased plasma levels of IL-17D, IL-17E and IL-17F were noted when comparing severe versus mild COVID-19. IL-17F, but not IL-17A, was significantly elevated in people with COVID-19 compared to healthy controls and with more severe disease. In vitro work on endothelial cells treated with IL-17F for 24h showed an increase cell surface expression of ICAM-1 accompanied by neutrophil adhesion. The introduction of two MAPK inhibitors significantly reduced the binding of neutrophils while also reducing ICAM-1 expression at the surface level of endothelial cells, but not its intracellular expression. Overall, these results have identified an association between two cytokines of the IL-17 family (IL-17D and IL-17F) with COVID-19 and disease severity. Considering that IL-17F stimulation promotes neutrophil adhesion to the endothelium in a MAPK-dependent manner, it is attractive to speculate that this pathway may contribute to pathogenic immunothrombosis in concert with other molecular effectors.

2024-10-17

Frontiers in Immunology (publié)

doi.org

Validation of ANG-1 and P-SEL as biomarkers of post-COVID-19 conditions using data from the Biobanque québécoise de la COVID-19 (BQC-19)

Eric Yamga

Antoine Soulé

Alain Piché

Amin Emad

Madeleine Durand

Simon Rousseau

2023-10-23

Clinical Proteomics (publié)