Turncoat antibodies unmasked in a model of autoimmune demyelination: from biology to therapy
Reza Taghipour-Mirakmahaleh
Françoise Morin
Yu Zhang
Louis Bourhoven
Louis-Charles Béland
Qun Zhou
Julie Jaworski
Anna Park
Juan Manuel Dominguez
Eoin P Flanagan
Romain Marignier
Catherine Larochelle
Steven Kerfoot
Luc Vallières
Autoantibodies contribute to many autoimmune diseases, yet there is no approved therapy to neutralize them selectively. A popular mouse mode… (see more)l, experimental autoimmune encephalomyelitis (EAE), could serve to develop such a therapy, provided we can better understand the nature and importance of the autoantibodies involved. Here we report the discovery of autoantibody-secreting extrafollicular plasmablasts in EAE induced with specific myelin oligodendrocyte glycoprotein (MOG) antigens. Single-cell RNA sequencing reveals that these cells produce non-affinity-matured IgG antibodies. These include pathogenic antibodies competing for shared binding space on MOG’s extracellular domain. Interestingly, the synthetic anti-MOG antibody 8-18C5 can prevent the binding of pathogenic antibodies from either EAE mice or people with MOG antibody disease (MOGAD). Moreover, an 8-18C5 variant carrying the NNAS mutation, which inactivates its effector functions, can reduce EAE severity and promote functional recovery. In brief, this study provides not only a comprehensive characterization of the humoral response in EAE models, but also a proof of concept for a novel therapy to antagonize pathogenic anti-MOG antibodies.
Assessing and Learning Alignment of Unimodal Vision and Language Models
Le Zhang
Qian Yang
How well are unimodal vision and language models aligned? Although prior work have approached answering this question, their assessment meth… (see more)ods do not directly translate to how these models are used in practical vision-language tasks. In this paper, we propose a direct assessment method, inspired by linear probing, to assess vision-language alignment. We identify that the degree of alignment of the SSL vision models depends on their SSL training objective, and we find that the clustering quality of SSL representations has a stronger impact on alignment performance than their linear separability. Next, we introduce Swift Alignment of Image and Language (SAIL), a efficient transfer learning framework that aligns pretrained unimodal vision and language models for downstream vision-language tasks. Since SAIL leverages the strengths of pretrained unimodal models, it requires significantly fewer (6%) paired image-text data for the multimodal alignment compared to models like CLIP which are trained from scratch. SAIL training only requires a single A100 GPU, 5 hours of training and can accommodate a batch size up to 32,768. SAIL achieves 73.4% zero-shot accuracy on ImageNet (vs. CLIP's 72.7%) and excels in zero-shot retrieval, complex reasoning, and semantic segmentation. Additionally, SAIL improves the language-compatibility of vision encoders that in turn enhance the performance of multimodal large language models. The entire codebase and model weights are open-source: https://lezhang7.github.io/sail.github.io/
Assessing and Learning Alignment of Unimodal Vision and Language Models
Le Zhang
Qian Yang
How well are unimodal vision and language models aligned? Although prior work have approached answering this question, their assessment meth… (see more)ods do not directly translate to how these models are used in practical vision-language tasks. In this paper, we propose a direct assessment method, inspired by linear probing, to assess vision-language alignment. We identify that the degree of alignment of the SSL vision models depends on their SSL training objective, and we find that the clustering quality of SSL representations has a stronger impact on alignment performance than their linear separability. Next, we introduce Swift Alignment of Image and Language (SAIL), a efficient transfer learning framework that aligns pretrained unimodal vision and language models for downstream vision-language tasks. Since SAIL leverages the strengths of pretrained unimodal models, it requires significantly fewer (6%) paired image-text data for the multimodal alignment compared to models like CLIP which are trained from scratch. SAIL training only requires a single A100 GPU, 5 hours of training and can accommodate a batch size up to 32,768. SAIL achieves 73.4% zero-shot accuracy on ImageNet (vs. CLIP's 72.7%) and excels in zero-shot retrieval, complex reasoning, and semantic segmentation. Additionally, SAIL improves the language-compatibility of vision encoders that in turn enhance the performance of multimodal large language models. The entire codebase and model weights are open-source: https://lezhang7.github.io/sail.github.io/
Assessing and Learning Alignment of Unimodal Vision and Language Models
Le Zhang
Qian Yang
How well are unimodal vision and language models aligned? Although prior work have approached answering this question, their assessment meth… (see more)ods do not directly translate to how these models are used in practical vision-language tasks. In this paper, we propose a direct assessment method, inspired by linear probing, to assess vision-language alignment. We identify that the degree of alignment of the SSL vision models depends on their SSL training objective, and we find that the clustering quality of SSL representations has a stronger impact on alignment performance than their linear separability. Next, we introduce Swift Alignment of Image and Language (SAIL), a efficient transfer learning framework that aligns pretrained unimodal vision and language models for downstream vision-language tasks. Since SAIL leverages the strengths of pretrained unimodal models, it requires significantly fewer (6%) paired image-text data for the multimodal alignment compared to models like CLIP which are trained from scratch. SAIL training only requires a single A100 GPU, 5 hours of training and can accommodate a batch size up to 32,768. SAIL achieves 73.4% zero-shot accuracy on ImageNet (vs. CLIP's 72.7%) and excels in zero-shot retrieval, complex reasoning, and semantic segmentation. Additionally, SAIL improves the language-compatibility of vision encoders that in turn enhance the performance of multimodal large language models. The entire codebase and model weights are open-source: https://lezhang7.github.io/sail.github.io/
BigDocs: An Open and Permissively-Licensed Dataset for Training Multimodal Models on Document and Code Tasks
Juan Rodriguez
Xiangru Jian
Siba Smarak Panigrahi
Tianyu Zhang
Aarash Feizi
Abhay Puri
Akshay Kalkunte
Franccois Savard
Ahmed Masry
Shravan Nayak
Rabiul Awal
Mahsa Massoud
Amirhossein Abaskohi
Zichao Li
Suyuchen Wang
Pierre-Andre Noel
Mats Leon Richter
Saverio Vadacchino
Shubbam Agarwal
Sanket Biswas … (see 23 more)
Sara Shanian
Ying Zhang
Noah Bolger
Kurt MacDonald
Simon Fauvel
Sathwik Tejaswi
Srinivas Sunkara
Joao Monteiro
Krishnamurthy Dj Dvijotham
Torsten Scholak
Sepideh Kharaghani
Sean Hughes
M. Özsu
Issam Hadj Laradji
Spandanna Gella
Perouz Taslakian
David Vazquez
Sai Rajeswar
Multimodal AI has the potential to significantly enhance document-understanding tasks, such as processing receipts, understanding workflows,… (see more) extracting data from documents, and summarizing reports. Code generation tasks that require long-structured outputs can also be enhanced by multimodality. Despite this, their use in commercial applications is often limited due to limited access to training data and restrictive licensing, which hinders open access. To address these limitations, we introduce BigDocs-7.5M, a high-quality, open-access dataset comprising 7.5 million multimodal documents across 30 tasks. We use an efficient data curation process to ensure our data is high-quality and license-permissive. Our process emphasizes accountability, responsibility, and transparency through filtering rules, traceable metadata, and careful content analysis. Additionally, we introduce BigDocs-Bench, a benchmark suite with 10 novel tasks where we create datasets that reflect real-world use cases involving reasoning over Graphical User Interfaces (GUI) and code generation from images. Our experiments show that training with BigDocs-Bench improves average performance up to 25.8% over closed-source GPT-4o in document reasoning and structured output tasks such as Screenshot2HTML or Image2Latex generation. Finally, human evaluations showed a preference for outputs from models trained on BigDocs over GPT-4o. This suggests that BigDocs can help both academics and the open-source community utilize and improve AI tools to enhance multimodal capabilities and document reasoning. The project is hosted at https://bigdocs.github.io .
BigDocs: An Open and Permissively-Licensed Dataset for Training Multimodal Models on Document and Code Tasks
Juan Rodriguez
Xiangru Jian
Siba Smarak Panigrahi
Tianyu Zhang
Aarash Feizi
Abhay Puri
Akshay Kalkunte
Franccois Savard
Ahmed Masry
Shravan Nayak
Rabiul Awal
Mahsa Massoud
Amirhossein Abaskohi
Zichao Li
Suyuchen Wang
Pierre-Andre Noel
M. L. Richter
Saverio Vadacchino
Shubbam Agarwal
Sanket Biswas … (see 23 more)
Sara Shanian
Ying Zhang
Noah Bolger
Kurt MacDonald
Simon Fauvel
Sathwik Tejaswi
Srinivas Sunkara
Joao Monteiro
Krishnamurthy Dj Dvijotham
Torsten Scholak
Sepideh Kharagani
Sean Hughes
M. Özsu
Issam Hadj Laradji
Spandanna Gella
Perouz Taslakian
David Vazquez
Sai Rajeswar
Multimodal AI has the potential to significantly enhance document-understanding tasks, such as processing receipts, understanding workflows,… (see more) extracting data from documents, and summarizing reports. Code generation tasks that require long-structured outputs can also be enhanced by multimodality. Despite this, their use in commercial applications is often limited due to limited access to training data and restrictive licensing, which hinders open access. To address these limitations, we introduce BigDocs-7.5M, a high-quality, open-access dataset comprising 7.5 million multimodal documents across 30 tasks. We use an efficient data curation process to ensure our data is high-quality and license-permissive. Our process emphasizes accountability, responsibility, and transparency through filtering rules, traceable metadata, and careful content analysis. Additionally, we introduce BigDocs-Bench, a benchmark suite with 10 novel tasks where we create datasets that reflect real-world use cases involving reasoning over Graphical User Interfaces (GUI) and code generation from images. Our experiments show that training with BigDocs-Bench improves average performance up to 25.8% over closed-source GPT-4o in document reasoning and structured output tasks such as Screenshot2HTML or Image2Latex generation. Finally, human evaluations showed a preference for outputs from models trained on BigDocs over GPT-4o. This suggests that BigDocs can help both academics and the open-source community utilize and improve AI tools to enhance multimodal capabilities and document reasoning. The project is hosted at https://bigdocs.github.io .
BigDocs: An Open and Permissively-Licensed Dataset for Training Multimodal Models on Document and Code Tasks
Juan Rodriguez
Xiangru Jian
Siba Smarak Panigrahi
Tianyu Zhang
Aarash Feizi
Abhay Puri
Akshay Kalkunte
Franccois Savard
Ahmed Masry
Shravan Nayak
Rabiul Awal
Mahsa Massoud
Amirhossein Abaskohi
Zichao Li
Suyuchen Wang
Pierre-Andre Noel
Mats Leon Richter
Saverio Vadacchino
Shubbam Agarwal
Sanket Biswas … (see 23 more)
Sara Shanian
Ying Zhang
Noah Bolger
Kurt MacDonald
Simon Fauvel
Sathwik Tejaswi
Srinivas Sunkara
Joao Monteiro
Krishnamurthy Dj Dvijotham
Torsten Scholak
Sepideh Kharaghani
Sean Hughes
M. Özsu
Issam Hadj Laradji
Spandanna Gella
Perouz Taslakian
David Vazquez
Sai Rajeswar
Multimodal AI has the potential to significantly enhance document-understanding tasks, such as processing receipts, understanding workflows,… (see more) extracting data from documents, and summarizing reports. Code generation tasks that require long-structured outputs can also be enhanced by multimodality. Despite this, their use in commercial applications is often limited due to limited access to training data and restrictive licensing, which hinders open access. To address these limitations, we introduce BigDocs-7.5M, a high-quality, open-access dataset comprising 7.5 million multimodal documents across 30 tasks. We use an efficient data curation process to ensure our data is high-quality and license-permissive. Our process emphasizes accountability, responsibility, and transparency through filtering rules, traceable metadata, and careful content analysis. Additionally, we introduce BigDocs-Bench, a benchmark suite with 10 novel tasks where we create datasets that reflect real-world use cases involving reasoning over Graphical User Interfaces (GUI) and code generation from images. Our experiments show that training with BigDocs-Bench improves average performance up to 25.8% over closed-source GPT-4o in document reasoning and structured output tasks such as Screenshot2HTML or Image2Latex generation. Finally, human evaluations showed a preference for outputs from models trained on BigDocs over GPT-4o. This suggests that BigDocs can help both academics and the open-source community utilize and improve AI tools to enhance multimodal capabilities and document reasoning. The project is hosted at https://bigdocs.github.io .
BigDocs: An Open and Permissively-Licensed Dataset for Training Multimodal Models on Document and Code Tasks
Juan Rodriguez
Xiangru Jian
Siba Smarak Panigrahi
Tianyu Zhang
Aarash Feizi
Abhay Puri
Akshay Kalkunte
Franccois Savard
Ahmed Masry
Shravan Nayak
Rabiul Awal
Mahsa Massoud
Amirhossein Abaskohi
Zichao Li
Suyuchen Wang
Pierre-Andre Noel
Mats Leon Richter
Saverio Vadacchino
Shubbam Agarwal
Sanket Biswas … (see 23 more)
Sara Shanian
Ying Zhang
Noah Bolger
Kurt MacDonald
Simon Fauvel
Sathwik Tejaswi
Srinivas Sunkara
Joao Monteiro
Krishnamurthy Dj Dvijotham
Torsten Scholak
Sepideh Kharaghani
Sean Hughes
M. Özsu
Issam Hadj Laradji
Spandanna Gella
Perouz Taslakian
David Vazquez
Sai Rajeswar
Multimodal AI has the potential to significantly enhance document-understanding tasks, such as processing receipts, understanding workflows,… (see more) extracting data from documents, and summarizing reports. Code generation tasks that require long-structured outputs can also be enhanced by multimodality. Despite this, their use in commercial applications is often limited due to limited access to training data and restrictive licensing, which hinders open access. To address these limitations, we introduce BigDocs-7.5M, a high-quality, open-access dataset comprising 7.5 million multimodal documents across 30 tasks. We use an efficient data curation process to ensure our data is high-quality and license-permissive. Our process emphasizes accountability, responsibility, and transparency through filtering rules, traceable metadata, and careful content analysis. Additionally, we introduce BigDocs-Bench, a benchmark suite with 10 novel tasks where we create datasets that reflect real-world use cases involving reasoning over Graphical User Interfaces (GUI) and code generation from images. Our experiments show that training with BigDocs-Bench improves average performance up to 25.8% over closed-source GPT-4o in document reasoning and structured output tasks such as Screenshot2HTML or Image2Latex generation. Finally, human evaluations showed a preference for outputs from models trained on BigDocs over GPT-4o. This suggests that BigDocs can help both academics and the open-source community utilize and improve AI tools to enhance multimodal capabilities and document reasoning. The project is hosted at https://bigdocs.github.io .
BigDocs: An Open and Permissively-Licensed Dataset for Training Multimodal Models on Document and Code Tasks
Juan Rodriguez
Xiangru Jian
Siba Smarak Panigrahi
Tianyu Zhang
Aarash Feizi
Abhay Puri
Akshay Kalkunte
Franccois Savard
Ahmed Masry
Shravan Nayak
Rabiul Awal
Mahsa Massoud
Amirhossein Abaskohi
Zichao Li
Suyuchen Wang
Pierre-Andre Noel
M. L. Richter
Saverio Vadacchino
Shubbam Agarwal
Sanket Biswas … (see 23 more)
Sara Shanian
Ying Zhang
Noah Bolger
Kurt MacDonald
Simon Fauvel
Sathwik Tejaswi
Srinivas Sunkara
Joao Monteiro
Krishnamurthy Dj Dvijotham
Torsten Scholak
Sepideh Kharagani
Sean Hughes
M. Özsu
Issam Hadj Laradji
Spandanna Gella
Perouz Taslakian
David Vazquez
Sai Rajeswar
Multimodal AI has the potential to significantly enhance document-understanding tasks, such as processing receipts, understanding workflows,… (see more) extracting data from documents, and summarizing reports. Code generation tasks that require long-structured outputs can also be enhanced by multimodality. Despite this, their use in commercial applications is often limited due to limited access to training data and restrictive licensing, which hinders open access. To address these limitations, we introduce BigDocs-7.5M, a high-quality, open-access dataset comprising 7.5 million multimodal documents across 30 tasks. We use an efficient data curation process to ensure our data is high-quality and license-permissive. Our process emphasizes accountability, responsibility, and transparency through filtering rules, traceable metadata, and careful content analysis. Additionally, we introduce BigDocs-Bench, a benchmark suite with 10 novel tasks where we create datasets that reflect real-world use cases involving reasoning over Graphical User Interfaces (GUI) and code generation from images. Our experiments show that training with BigDocs-Bench improves average performance up to 25.8% over closed-source GPT-4o in document reasoning and structured output tasks such as Screenshot2HTML or Image2Latex generation. Finally, human evaluations showed a preference for outputs from models trained on BigDocs over GPT-4o. This suggests that BigDocs can help both academics and the open-source community utilize and improve AI tools to enhance multimodal capabilities and document reasoning. The project is hosted at https://bigdocs.github.io .
CellSexID: Sex-Based Computational Tracking of Cellular Origins in Chimeric Models
Huilin Tai
Qian Li
Jingtao Wang
Jiahui Tan
Ryann Lang
Basil J. Petrof
Cell tracking in chimeric models is essential yet challenging, particularly in developmental biology, regenerative medicine, and transplanta… (see more)tion studies. Existing methods, such as fluorescent labeling and genetic barcoding, are technically demanding, costly, and often impractical for dynamic, heterogeneous tissues. To address these limitations, we propose a computational framework that leverages sex as a surrogate marker for cell tracking. Our approach uses a machine learning model trained on single-cell transcriptomic data to predict cell sex with high accuracy, enabling clear distinction between donor (male) and recipient (female) cells in sex-mismatched chimeric models. The model identifies specific genes critical for sex prediction and has been validated using public datasets and experimental flow sorting, confirming the biological relevance of the identified cell populations. Applied to skeletal muscle macrophages, our method revealed distinct transcriptional profiles associated with cellular origins. This pipeline offers a robust, cost-effective solution for cell tracking in chimeric models, advancing research in regenerative medicine and immunology by providing precise insights into cellular origins and therapeutic outcomes.
CellSexID: Sex-Based Computational Tracking of Cellular Origins in Chimeric Models
Huilin Tai
Qian Li
Jingtao Wang
Jiahui Tan
Ryann Lang
Basil J. Petrof
Cell tracking in chimeric models is essential yet challenging, particularly in developmental biology, regenerative medicine, and transplanta… (see more)tion studies. Existing methods, such as fluorescent labeling and genetic barcoding, are technically demanding, costly, and often impractical for dynamic, heterogeneous tissues. To address these limitations, we propose a computational framework that leverages sex as a surrogate marker for cell tracking. Our approach uses a machine learning model trained on single-cell transcriptomic data to predict cell sex with high accuracy, enabling clear distinction between donor (male) and recipient (female) cells in sex-mismatched chimeric models. The model identifies specific genes critical for sex prediction and has been validated using public datasets and experimental flow sorting, confirming the biological relevance of the identified cell populations. Applied to skeletal muscle macrophages, our method revealed distinct transcriptional profiles associated with cellular origins. This pipeline offers a robust, cost-effective solution for cell tracking in chimeric models, advancing research in regenerative medicine and immunology by providing precise insights into cellular origins and therapeutic outcomes.
An Efficient Model Maintenance Approach for MLOps
Forough Majidi
Heng Li
Amin Nikanjam
In recent years, many industries have utilized machine learning models (ML) in their systems. Ideally, machine learning models should be tra… (see more)ined on and applied to data from the same distributions. However, the data evolves over time in many application areas, leading to data and concept drift, which in turn causes the performance of the ML models to degrade over time. Therefore, maintaining up to date ML models plays a critical role in the MLOps pipeline. Existing ML model maintenance approaches are often computationally resource intensive, costly, time consuming, and model dependent. Thus, we propose an improved MLOps pipeline, a new model maintenance approach and a Similarity Based Model Reuse (SimReuse) tool to address the challenges of ML model maintenance. We identify seasonal and recurrent distribution patterns in time series datasets throughout a preliminary study. Recurrent distribution patterns enable us to reuse previously trained models for similar distributions in the future, thus avoiding frequent retraining. Then, we integrated the model reuse approach into the MLOps pipeline and proposed our improved MLOps pipeline. Furthermore, we develop SimReuse, a tool to implement the new components of our MLOps pipeline to store models and reuse them for inference of data segments with similar data distributions in the future. Our evaluation results on four time series datasets demonstrate that our model reuse approach can maintain the performance of models while significantly reducing maintenance time and costs. Our model reuse approach achieves ML performance comparable to the best baseline, while being 15 times more efficient in terms of computation time and costs. Therefore, industries and practitioners can benefit from our approach and use our tool to maintain the performance of their ML models in the deployment phase to reduce their maintenance costs.